Fig. 4
From: Implication of system xc− in neuroinflammation during the onset and maintenance of neuropathic pain
Assessment of spinal mRNA of glial markers following PSNL or sham surgery in xCT+/+ and xCT−/− mice. Three (left panels) and seven (right panels) days post-surgery, the ipsilateral and contralateral quadrants of the dorsal horn of the lumbar spinal cord were dissected and used for RT-qPCR analyses. Specific markers were used to evaluate astrocytic (A, B) and microglial (C, D) activation, GFAP and Iba-1 respectively. Other markers were used to assess microglia activation profile towards pro-inflammatory (NOX-2: panel E & F) and anti-inflammatory (Arg-1: panel G & H) phenotype. For all markers, the mRNA was quantified, normalized to the mean of 3 housekeeping genes (RPL-19, Ywhaz, HPRT-1) and expressed as a percentage of the respective contralateral mRNA level. As shown on the figure, data were obtained from 5 to 7 different animals per group and are presented as geometric mean with SD. Statistical analyses were performed through a two-way ANOVA model. Main and interaction effects are indicated below the graphs. When statistically significant interaction was detected, Tukey’s pairwise comparisons were conducted. P-values for significant effects are reported on the graphs. PSNL partial sciatic nerve ligation; GFAP glial fibrillary acidic protein; Iba-1 ionized calcium binding adapter protein 1; NOX-2 NADPH oxidase 2; Arg-1 Arginase 1; S surgery effect; G genotype effect; S*G interaction effect