Fig. 3
From: Implication of system xc− in neuroinflammation during the onset and maintenance of neuropathic pain
Glial activation in the spinal cord following PSNL or sham surgery in xCT+/+ and xCT−/− mice. Immunohistological stainings for GFAP and Iba-1 were analyzed 3 (A, B, D) and 7 (A, C, E) days after the surgery in samples from wild-type or transgenic mice lacking xCT (A). The fluorescent intensity for GFAP (B, C) and Iba-1 (D, E) in the dorsal horns was quantified. The results from the ipsilateral side are normalized and expressed as a percentage of the respective contralateral side. Images shown are representative of 5 different animals and histograms represent the geometric means with SD. Statistical analyses were performed through a two-way ANOVA model. Main and interaction effects are indicated below the graphs. When statistically significant interaction was detected, Tukey’s pairwise comparisons were conducted. P-values for significant effects are reported on the graphs. PSNL partial sciatic nerve ligation; GFAP glial fibrillary acidic protein; Iba-1 ionized calcium binding adapter protein 1; S surgery effect; G genotype effect; S*G interaction effect