Fig. 2
From: Implication of system xc− in neuroinflammation during the onset and maintenance of neuropathic pain
Allodynia and hyperalgesia in xCT+/+ and xCT−/− mice following PSNL or sham surgery. Pain hypersensitivity was assessed upon stimulation of the ipsilateral (A, B, D, E) and contralateral (C, F) hind paw at baseline (BL) and for up to 7 days after surgery. The 50% PWT to mechanical stimulation was used to determine allodynia (A-C), whereas the PWL to thermal stimulation was used as a read-out of hyperalgesia (D-F). Panel B and E depict the area under the curve (AUC in arbitrary unit a.u.) of the ipsilateral 50% PWT and PWL, respectively. Data are presented as arithmetic mean with SEM (A, C, D, F) or geometric mean with SD (B, E) of 5 animals per group for allodynia and 7 animals per group for hyperalgesia. Statistical analyses were performed through a two-way ANOVA model (B, E) with repeated measures (A, C, D, F). Main and interaction effects are indicated below the graphs. When statistically significant interaction was detected, Tukey’s pairwise comparisons were conducted. P-values for significant effects are reported on the graphs (B, E). In panel A and D, * indicates a significant difference between lesioned wild-type (PSNL xCT+/+) and transgenic mice lacking xCT (PSNL xCT−/−), # indicate a significant difference between lesioned and non-lesioned animals (within the same genotype). PSNL partial sciatic nerve ligation; PWT paw withdrawal threshold; PWL paw withdrawal latency; AUC area under the curve; S surgery effect; G genotype effect; S*G interaction effect