Fig. 1
From: Implication of system xc− in neuroinflammation during the onset and maintenance of neuropathic pain
xCT expression and activity in the lumbar dorsal spinal cord following PSNL or sham surgery. Three (panel A & B) and seven (panel C & D) days post-surgery, the ipsilateral and contralateral dorsal horn quadrants of the lumbar spinal cord were dissected and used for RT-qPCR or functional assay. xCT mRNA was quantified, normalized to the mean of 3 housekeeping genes (RPL-19, Ywhaz, HPRT-1) and expressed as a percentage of the respective contralateral mRNA level (A, C). Uptake of 3H-L-glutamate on crude spinal synaptosomes was used to determine system xc− functionality (B, D). Ipsilateral system xc− specific uptake (HCA-dependent) is expressed as relative (%) to the respective contralateral side. As shown on the figure, data were obtained from 5 to 7 different animals per group and are presented as geometric mean with SD. Statistical analyses were conducted using a one-way ANOVA model. PSNL partial sciatic nerve ligation; Glu glutamate; HCA homocysteic acid