Fig. 2
TBI exacerbates HFD-induced increases in VAT inflammatory responses. A The effect of HFD and TBI on VAT weight (g/g BW) at 28 dpi. B TBI-HFD mice display a significant increase in CD45+ and CD11b+ C ATM/myeloid cells when compared to Sham-HFD counterparts. Representative dot plot for % CD45+/CD11b+ ATM/myeloid cells D. TBI-HFD significantly increased phagocytotic (Bead+CD11b+ myeloid) cell number when compared to Sham-HFD counterparts E. Neither TBI nor HFD impacted the number of CD11b− lymphocyte cell population F. Sham-HFD mice display a significant increase in VAT expression of TNF-α, NLRP3, p22phox, and IL-10, when compared to Sham-SD counterparts G. TBI-HFD mice exhibit a significant increase in VAT expression of IL-1β and NLRP3, when compared to TBI-SD counterparts and notably compared to Sham-HFD in the case of NLRP3. * p < 0.05, ** p < 0.01, *** p < 0.001 vs Sham-SD; +  +  +  + p < 0.0001 vs TBI-SD; ## p < 0.01 vs Sham-HFD. N = 6–7 per group. Data expressed as mean ± SEM