Fig. 3

DMF partly reversed the proportional and transcriptional alterations in T-cell subsets during EAU. A. UMAP plots of T-cell subsets from the CDLNs of all the mouse groups. B. UMAP plots of canonical markers of T-cell subsets from all the mouse groups. C. Bar plots showing the percentages of T-cell subsets among CDLNs from normal, EAU, and DMF-treated EAU mice. D. Pseudotime trajectory analysis of CD4 + T cells. Cells are colored according to pseudotime (left) or cell type (right). E-F. Line plots showing the expression of Pim1 (E) and Cxcr4 (F) in each CD4 + T-cell subset. G. Rose diagram showing the numbers of upregulated and downregulated rescue DEGs in each T-cell subset. H. Dot plot showing GO terms enriched by downregulated rescue DEGs in NCD4, Tfh, Th1, Th17, Treg, Prot, Ctl, and NCD8 cells. I. Dot plot showing GO terms enriched by upregulated rescue DEGs in NCD4, Th1, Th17, Treg, and NCD8 cells