Fig. 4

PD depletes macrophages and DCs in secondary lymphoid organs (SLOs) in preclinical EAE. Pooled spleen and draining lymph nodes (SLOs) were analyzed before clinical symptom onset (preonset EAE). A Numbers of total cells, and of myeloid (CD45+CD11b+) and lymphocytic (CD45+CD11b-) cells were lower in the SLOs of PD compared to those of CD mice. B Gating strategy for flow cytometry analysis of SLO myeloid cells in EAE. C Neutrophil (CD45+CD11b+Ly6G+) frequencies were increased but not their numbers in PD mice compared to CD mice. D Frequencies of all monocytes (CD45+CD11b+Ly6G-CD11c-MHCII-) were increased but their numbers were decreased in SLOs of PD compared to those of CD mice. Both frequencies and numbers of total DCs (CD45+CD11b+Ly6G-CD11c+MHCII+), and of total macrophages (CD45+CD11b+Ly6G-CD11c-MHCII+) were decreased in PD compared to CD. E Frequencies of classical DCs (cDCs; CD45+CD11b+Ly6G-CD11c+MHCII+CD26+CD88-) were increased, and those of monocyte-derived DCs (moDCs; CD45+CD11b+Ly6G-CD11c+MHCII+CD26-CD88+) were decreased but the absolute numbers of both subsets were decreased in the PD SLOs compared to CD. F Ly6C+ monocyte and macrophage subsets (CD45+CD11b+Ly6G-CD11c-MHCII+Ly6C+) were decreased in the PD SLOs compared to those of CD mice. Representative flow plots are shown. Both male and female mice were employed. Data are shown as means ± SD, n = 22; *p < 0.05, **p < 0.005, ****p < 0.00005