Figure 6

Effects of COX-2 deficiency on LPS-induced expression of transcription factors NF-κB and STAT3, and SOCS3. Quantitative real time-PCR analysis of NF-κB (A), SOCS3 (B) and STAT3 mRNA (C) for COX-2^+/+ and COX-2^-/- mice 24 h after icv injection of LPS or vehicle. (D) Quantification of phosphorylated STAT3 (Tyr 705) protein levels, relative to the total STAT3 in the brain nuclear fraction. (E) Representative immunoblot of P-STAT3 expression in COX-2^+/+ and COX-2^-/- mice 24 h after icv injection of LPS or vehicle. Data are presented as mean ± SEM (n = 4-6). *P < 0.05, **P < 0.01, ***P < 0.001 compared to the corresponding vehicle-injected mice; ^##P < 0.01 compared to the LPS-injected COX-2^+/+ mice.